How deep should I sequence Curio Seeker libraries?

Although we recommend 200-600 million paired-end reads for the 3x3 mm tile and 2-3 billion paired-end reads for the 10x10 mm tile, deeper sequencing may be required for more transcriptionally active tissue types. In the html output file from the Curio bioinformatics pipeline, the metric "median reads/UMI" indicates the approximate sequencing saturation. We recommend a value of 8-10 to reach saturation. If it is lower than this range, we recommend sequencing more to recover additional UMIs.