Sequencing
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      2. Curio Seeker
      3. Sequencing

      How much PhiX should I spike in to my library before sequencing?

      • NextSeq 1000/2000: 5% PhiX spike-in
      • NextSeq 500/550: 5% PhiX spike-in
      • NovaSeq 6000: 

           - 5% PhiX spike-in when pooling with non-Curio Seeker libraries 
           - 10% PhiX spike-in when pooling with only Curio Seeker libraries